Primers Available
Organism:Sirthenea flavipes mitochondrion
Repeat:(ATCA)3
Start:763 End:774
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| TAGCAACCATTATTGGGGCA | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| CATGCATGCTACAATTCATCC | |||||||||||||
| Tm_F_1 | 61.207 | Size_F_1 | 20 | Tm_R_1 | 59.008 | Size_R_1 | 21 | PRODUCT Size | 104 bp | Primer Start | 695 | Primer End | 798 |
| FORWARD_PRIMER_2 | |||||||||||||
| CCATTTCATTTCTGATTCCCA | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| CATGCATGCTACAATTCATCC | |||||||||||||
| Tm_F_2 | 59.748 | Size_F_2 | 21 | Tm_R_2 | 59.008 | Size_R_2 | 21 | PRODUCT Size | 249 bp | Primer Start | 550 | Primer End | 798 |
| FORWARD_PRIMER_3 | |||||||||||||
| CCCCCATTTCATTTCTGATT | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| CATGCATGCTACAATTCATCC | |||||||||||||
| Tm_F_3 | 58.69 | Size_F_3 | 20 | Tm_R_3 | 59.008 | Size_R_3 | 21 | PRODUCT Size | 252 bp | Primer Start | 547 | Primer End | 798 |