Primers Available
Organism:Microcaecilia sp. PZ-2009 mitochondrion
Repeat:(T)10
Start:15639 End:15648
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| TTCGAGAACGTCCCAGCTAT | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| TGCATGAAGCAATCCAACTT | |||||||||||||
| Tm_F_1 | 59.836 | Size_F_1 | 20 | Tm_R_1 | 59.276 | Size_R_1 | 20 | PRODUCT Size | 257 bp | Primer Start | 15458 | Primer End | 15714 |
| FORWARD_PRIMER_2 | |||||||||||||
| CGAGAACGTCCCAGCTATGT | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| TGCATGAAGCAATCCAACTT | |||||||||||||
| Tm_F_2 | 60.277 | Size_F_2 | 20 | Tm_R_2 | 59.276 | Size_R_2 | 20 | PRODUCT Size | 255 bp | Primer Start | 15460 | Primer End | 15714 |
| FORWARD_PRIMER_3 | |||||||||||||
| TCCTGCAAACCTTCGAGAAC | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| TGCATGAAGCAATCCAACTT | |||||||||||||
| Tm_F_3 | 60.375 | Size_F_3 | 20 | Tm_R_3 | 59.276 | Size_R_3 | 20 | PRODUCT Size | 268 bp | Primer Start | 15447 | Primer End | 15714 |