Primers Available
Organism:Microcaecilia sp. PZ-2009 mitochondrion
Repeat:(CAAC)3
Start:8306 End:8317
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| TGGCCAAAAATGAGCACTAA | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| GCCTAATGCTATTGGTCGGA | |||||||||||||
| Tm_F_1 | 59.301 | Size_F_1 | 20 | Tm_R_1 | 60.06 | Size_R_1 | 20 | PRODUCT Size | 280 bp | Primer Start | 8143 | Primer End | 8422 |
| FORWARD_PRIMER_2 | |||||||||||||
| TACATGGCCAAAAATGAGCA | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| GGTGGGATAAGTGGAGTTGG | |||||||||||||
| Tm_F_2 | 60.073 | Size_F_2 | 20 | Tm_R_2 | 59.259 | Size_R_2 | 20 | PRODUCT Size | 232 bp | Primer Start | 8139 | Primer End | 8370 |
| FORWARD_PRIMER_3 | |||||||||||||
| TACATGGCCAAAAATGAGCA | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| GGTGGGATAAGTGGAGTTGGT | |||||||||||||
| Tm_F_3 | 60.073 | Size_F_3 | 20 | Tm_R_3 | 60.103 | Size_R_3 | 21 | PRODUCT Size | 232 bp | Primer Start | 8139 | Primer End | 8370 |