Primers Available
Organism:Microcaecilia sp. PZ-2009 mitochondrion
Repeat:(A)10
Start:5194 End:5203
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| TTCTGGACAGGTGAGCCTCT | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| CGGATTGTAGCTCCGAAAAC | |||||||||||||
| Tm_F_1 | 59.986 | Size_F_1 | 20 | Tm_R_1 | 59.708 | Size_R_1 | 20 | PRODUCT Size | 221 bp | Primer Start | 5105 | Primer End | 5325 |
| FORWARD_PRIMER_2 | |||||||||||||
| TTCTGGACAGGTGAGCCTCT | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| ACGTACATGCAGGAGTGCAA | |||||||||||||
| Tm_F_2 | 59.986 | Size_F_2 | 20 | Tm_R_2 | 60.333 | Size_R_2 | 20 | PRODUCT Size | 161 bp | Primer Start | 5105 | Primer End | 5265 |
| FORWARD_PRIMER_3 | |||||||||||||
| CTACAATCCACTTCTCCCGC | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| TGCCGATGTCTTTGTGGTTA | |||||||||||||
| Tm_F_3 | 59.694 | Size_F_3 | 20 | Tm_R_3 | 60.111 | Size_R_3 | 20 | PRODUCT Size | 229 bp | Primer Start | 5171 | Primer End | 5399 |