Primers Available
Organism:Caecilia volcani mitochondrion
Repeat:(AC)6
Start:419 End:430
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| CGGTTAAACAAGAAGCCCAA | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| GGTCGTGATGTGGGGTAATC | |||||||||||||
| Tm_F_1 | 60.103 | Size_F_1 | 20 | Tm_R_1 | 60.057 | Size_R_1 | 20 | PRODUCT Size | 168 bp | Primer Start | 307 | Primer End | 474 |
| FORWARD_PRIMER_2 | |||||||||||||
| ATTCACCCGGCTGTAGTACG | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| TTTGTGTTTTAGCGGTCGTG | |||||||||||||
| Tm_F_2 | 60.015 | Size_F_2 | 20 | Tm_R_2 | 59.769 | Size_R_2 | 20 | PRODUCT Size | 103 bp | Primer Start | 385 | Primer End | 487 |
| FORWARD_PRIMER_3 | |||||||||||||
| ATTCACCCGGCTGTAGTACG | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| GTGGTGAGGTAAAACGTGGG | |||||||||||||
| Tm_F_3 | 60.015 | Size_F_3 | 20 | Tm_R_3 | 60.269 | Size_R_3 | 20 | PRODUCT Size | 270 bp | Primer Start | 385 | Primer End | 654 |