Primers Available
Organism:Puntius semifasciolatus mitochondrion
Repeat:(TTA)4
Start:10748 End:10759
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| CACATTAACCCAGAGCCGAT | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| TCCTGCATTGAGTCGTTCAG | |||||||||||||
| Tm_F_1 | 59.955 | Size_F_1 | 20 | Tm_R_1 | 59.984 | Size_R_1 | 20 | PRODUCT Size | 192 bp | Primer Start | 10605 | Primer End | 10796 |
| FORWARD_PRIMER_2 | |||||||||||||
| ATCACATTAACCCAGAGCCG | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| TCCTGCATTGAGTCGTTCAG | |||||||||||||
| Tm_F_2 | 59.955 | Size_F_2 | 20 | Tm_R_2 | 59.984 | Size_R_2 | 20 | PRODUCT Size | 194 bp | Primer Start | 10603 | Primer End | 10796 |
| FORWARD_PRIMER_3 | |||||||||||||
| CACATTAACCCAGAGCCGAT | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| ATGTGCCTGTGGATTGTTGA | |||||||||||||
| Tm_F_3 | 59.955 | Size_F_3 | 20 | Tm_R_3 | 59.967 | Size_R_3 | 20 | PRODUCT Size | 274 bp | Primer Start | 10605 | Primer End | 10878 |