Primers Available
Organism:Puntius semifasciolatus mitochondrion
Repeat:(CAAA)3
Start:1517 End:1528
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| GCTCCGGGTAGAAGTGACAG | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| TTAGGTGGCTGCTTTTAGGC | |||||||||||||
| Tm_F_1 | 59.867 | Size_F_1 | 20 | Tm_R_1 | 59.496 | Size_R_1 | 20 | PRODUCT Size | 239 bp | Primer Start | 1427 | Primer End | 1665 |
| FORWARD_PRIMER_2 | |||||||||||||
| AGCTCCGGGTAGAAGTGACA | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| TTAGGTGGCTGCTTTTAGGC | |||||||||||||
| Tm_F_2 | 59.867 | Size_F_2 | 20 | Tm_R_2 | 59.496 | Size_R_2 | 20 | PRODUCT Size | 240 bp | Primer Start | 1426 | Primer End | 1665 |
| FORWARD_PRIMER_3 | |||||||||||||
| ACCGAACCTGGTGATAGCTG | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| TTAGGTGGCTGCTTTTAGGC | |||||||||||||
| Tm_F_3 | 60.134 | Size_F_3 | 20 | Tm_R_3 | 59.496 | Size_R_3 | 20 | PRODUCT Size | 215 bp | Primer Start | 1451 | Primer End | 1665 |