Primers Available
Organism:Puntius semifasciolatus mitochondrion
Repeat:(AT)19
Start:16458 End:16495
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| TGCTATCCTTGTCAAACCCC | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| GGCTAAAATAAATAAGTGGCGAA | |||||||||||||
| Tm_F_1 | 59.933 | Size_F_1 | 20 | Tm_R_1 | 58.806 | Size_R_1 | 23 | PRODUCT Size | 154 bp | Primer Start | 16377 | Primer End | 16530 |
| FORWARD_PRIMER_2 | |||||||||||||
| CTATCCTTGTCAAACCCCGA | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| GGCTAAAATAAATAAGTGGCGAA | |||||||||||||
| Tm_F_2 | 59.926 | Size_F_2 | 20 | Tm_R_2 | 58.806 | Size_R_2 | 23 | PRODUCT Size | 152 bp | Primer Start | 16379 | Primer End | 16530 |
| FORWARD_PRIMER_3 | |||||||||||||
| CCAAGGAGGACTCAAGAACG | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| GGCTAAAATAAATAAGTGGCGAA | |||||||||||||
| Tm_F_3 | 59.837 | Size_F_3 | 20 | Tm_R_3 | 58.806 | Size_R_3 | 23 | PRODUCT Size | 130 bp | Primer Start | 16401 | Primer End | 16530 |