Primers Available
Organism:Bufo tibetanus mitochondrion
Repeat:(CCCT)3
Start:11472 End:11483
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| TGGCTCCTCGCTACTCTCAT | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| GATGTGTTCTCGGGTGTGTG | |||||||||||||
| Tm_F_1 | 60.119 | Size_F_1 | 20 | Tm_R_1 | 60.005 | Size_R_1 | 20 | PRODUCT Size | 231 bp | Primer Start | 11443 | Primer End | 11673 |
| FORWARD_PRIMER_2 | |||||||||||||
| TGGCTCCTCGCTACTCTCAT | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| GAGGATGTGTTCTCGGGTGT | |||||||||||||
| Tm_F_2 | 60.119 | Size_F_2 | 20 | Tm_R_2 | 59.969 | Size_R_2 | 20 | PRODUCT Size | 234 bp | Primer Start | 11443 | Primer End | 11676 |
| FORWARD_PRIMER_3 | |||||||||||||
| TGGCTCCTCGCTACTCTCAT | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| TTAAGTGGGATGGAAGGTGC | |||||||||||||
| Tm_F_3 | 60.119 | Size_F_3 | 20 | Tm_R_3 | 59.933 | Size_R_3 | 20 | PRODUCT Size | 196 bp | Primer Start | 11443 | Primer End | 11638 |