Primers Available
Organism:Epinephelus fuscoguttatus mitochondrion
Repeat:(CCAA)3
Start:1108 End:1119
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| CTTTCATCAGCAAAGGGGAG | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| TCCTTTTCTGTCGCCCATAC | |||||||||||||
| Tm_F_1 | 59.807 | Size_F_1 | 20 | Tm_R_1 | 60.074 | Size_R_1 | 20 | PRODUCT Size | 255 bp | Primer Start | 955 | Primer End | 1209 |
| FORWARD_PRIMER_2 | |||||||||||||
| CTTTCATCAGCAAAGGGGAG | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| GCCCATACTAAGGGGGAAAA | |||||||||||||
| Tm_F_2 | 59.807 | Size_F_2 | 20 | Tm_R_2 | 60.143 | Size_R_2 | 20 | PRODUCT Size | 243 bp | Primer Start | 955 | Primer End | 1197 |
| FORWARD_PRIMER_3 | |||||||||||||
| CTTTCATCAGCAAAGGGGAG | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| CTCTATCGCTCCGTTGTTCC | |||||||||||||
| Tm_F_3 | 59.807 | Size_F_3 | 20 | Tm_R_3 | 59.836 | Size_R_3 | 20 | PRODUCT Size | 272 bp | Primer Start | 955 | Primer End | 1226 |