Primers Available
Organism:Kaloula borealis mitochondrion
Repeat:(CTT)4
Start:5909 End:5920
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| AAAACCCCCATCAGTAACCC | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| GGGTGTCCGAAGAATCAGAA | |||||||||||||
| Tm_F_1 | 59.919 | Size_F_1 | 20 | Tm_R_1 | 60.05 | Size_R_1 | 20 | PRODUCT Size | 210 bp | Primer Start | 5841 | Primer End | 6050 |
| FORWARD_PRIMER_2 | |||||||||||||
| CCTGTTCGTCTGATCCGTTT | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| GTAGACCTCTGGGTGTCCGA | |||||||||||||
| Tm_F_2 | 60.111 | Size_F_2 | 20 | Tm_R_2 | 60.112 | Size_R_2 | 20 | PRODUCT Size | 187 bp | Primer Start | 5874 | Primer End | 6060 |
| FORWARD_PRIMER_3 | |||||||||||||
| CTCTTCAATTCTTGGGGCAA | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| GTAGACCTCTGGGTGTCCGA | |||||||||||||
| Tm_F_3 | 60.184 | Size_F_3 | 20 | Tm_R_3 | 60.112 | Size_R_3 | 20 | PRODUCT Size | 268 bp | Primer Start | 5793 | Primer End | 6060 |