Primers Available
Organism:Cnemaspis limi mitochondrion
Repeat:(CTA)4
Start:13505 End:13516
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| ACTAATACACCGCCACAGCC | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| GCTTGAATTAACGCGTGGTT | |||||||||||||
| Tm_F_1 | 60.022 | Size_F_1 | 20 | Tm_R_1 | 60.138 | Size_R_1 | 20 | PRODUCT Size | 271 bp | Primer Start | 13320 | Primer End | 13590 |
| FORWARD_PRIMER_2 | |||||||||||||
| ACCACAAACATCCGCCTATC | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| GCTTGAATTAACGCGTGGTT | |||||||||||||
| Tm_F_2 | 59.82 | Size_F_2 | 20 | Tm_R_2 | 60.138 | Size_R_2 | 20 | PRODUCT Size | 162 bp | Primer Start | 13429 | Primer End | 13590 |
| FORWARD_PRIMER_3 | |||||||||||||
| ACATCCGCCTATCAAACACC | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| GCTTGAATTAACGCGTGGTT | |||||||||||||
| Tm_F_3 | 59.82 | Size_F_3 | 20 | Tm_R_3 | 60.138 | Size_R_3 | 20 | PRODUCT Size | 155 bp | Primer Start | 13436 | Primer End | 13590 |