Primers Available
Organism:Phyllodactylus unctus mitochondrion
Repeat:(CGCC)3
Start:552 End:563
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| CTAGAACAGCACTTTGGCCC | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| TCGATTGTAGGACAGGCTCC | |||||||||||||
| Tm_F_1 | 59.875 | Size_F_1 | 20 | Tm_R_1 | 60.218 | Size_R_1 | 20 | PRODUCT Size | 268 bp | Primer Start | 377 | Primer End | 644 |
| FORWARD_PRIMER_2 | |||||||||||||
| AAGAACCCCCTCCCTAAACC | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| TCGATTGTAGGACAGGCTCC | |||||||||||||
| Tm_F_2 | 60.528 | Size_F_2 | 20 | Tm_R_2 | 60.218 | Size_R_2 | 20 | PRODUCT Size | 205 bp | Primer Start | 440 | Primer End | 644 |
| FORWARD_PRIMER_3 | |||||||||||||
| CTAGAACAGCACTTTGGCCC | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| GCATCGATTGTAGGACAGGC | |||||||||||||
| Tm_F_3 | 59.875 | Size_F_3 | 20 | Tm_R_3 | 60.634 | Size_R_3 | 20 | PRODUCT Size | 271 bp | Primer Start | 377 | Primer End | 647 |