Primers Available
Organism:Phyllodactylus unctus mitochondrion
Repeat:(ACCTAC)5(ACCCAT)5acccgtacctatacccatacccatacct(ATACCC)4atacct(ATACCC)4atacct(ATACCC)3
Start:16366 End:16531
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| CACACTCACCCACCCACATA | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| CGGGTATGGGTATAGGTACGG | |||||||||||||
| Tm_F_1 | 60.285 | Size_F_1 | 20 | Tm_R_1 | 60.33 | Size_R_1 | 21 | PRODUCT Size | 228 bp | Primer Start | 16340 | Primer End | 16567 |
| FORWARD_PRIMER_2 | |||||||||||||
| CACACTCACCCACCCACATA | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| AAAACGCGCGTATGGGTAT | |||||||||||||
| Tm_F_2 | 60.285 | Size_F_2 | 20 | Tm_R_2 | 60.358 | Size_R_2 | 19 | PRODUCT Size | 276 bp | Primer Start | 16340 | Primer End | 16615 |
| FORWARD_PRIMER_3 | |||||||||||||
| CACACTCACCCACCCACATA | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| AAAAACGCGCGTATGGGTAT | |||||||||||||
| Tm_F_3 | 60.285 | Size_F_3 | 20 | Tm_R_3 | 61.562 | Size_R_3 | 20 | PRODUCT Size | 277 bp | Primer Start | 16340 | Primer End | 16616 |