Primers Available
Organism:Heleophryne regis mitochondrion
Repeat:(TTC)4
Start:9754 End:9765
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| TCGGCCTCATCCTCATATTC | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| GGGTCAGCATTCACCAATTT | |||||||||||||
| Tm_F_1 | 59.998 | Size_F_1 | 20 | Tm_R_1 | 59.797 | Size_R_1 | 20 | PRODUCT Size | 218 bp | Primer Start | 9613 | Primer End | 9830 |
| FORWARD_PRIMER_2 | |||||||||||||
| ATTCAATGGTGACGGGATGT | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| GGGTCAGCATTCACCAATTT | |||||||||||||
| Tm_F_2 | 60.057 | Size_F_2 | 20 | Tm_R_2 | 59.797 | Size_R_2 | 20 | PRODUCT Size | 189 bp | Primer Start | 9642 | Primer End | 9830 |
| FORWARD_PRIMER_3 | |||||||||||||
| TCCAAAAGGGACTGCGATAC | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| GAATAGCATCTTTGCGGGAG | |||||||||||||
| Tm_F_3 | 60.074 | Size_F_3 | 20 | Tm_R_3 | 59.807 | Size_R_3 | 20 | PRODUCT Size | 258 bp | Primer Start | 9703 | Primer End | 9960 |