Primers Available
Organism:Heleophryne regis mitochondrion
Repeat:(C)13
Start:513 End:525
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| GGCCCTCATTATAAAGCCAA | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| GTTAAGCTACGTGGGCGTGT | |||||||||||||
| Tm_F_1 | 59.035 | Size_F_1 | 20 | Tm_R_1 | 60.197 | Size_R_1 | 20 | PRODUCT Size | 129 bp | Primer Start | 484 | Primer End | 612 |
| FORWARD_PRIMER_2 | |||||||||||||
| TTGGCCCTCATTATAAAGCC | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| GTTAAGCTACGTGGGCGTGT | |||||||||||||
| Tm_F_2 | 59.035 | Size_F_2 | 20 | Tm_R_2 | 60.197 | Size_R_2 | 20 | PRODUCT Size | 131 bp | Primer Start | 482 | Primer End | 612 |
| FORWARD_PRIMER_3 | |||||||||||||
| GGCCCTCATTATAAAGCCAA | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| TGATTAGGGGTGTAAGGGCA | |||||||||||||
| Tm_F_3 | 59.035 | Size_F_3 | 20 | Tm_R_3 | 60.323 | Size_R_3 | 20 | PRODUCT Size | 276 bp | Primer Start | 484 | Primer End | 759 |