Primers Available
Organism:Heleophryne regis mitochondrion
Repeat:(C)10
Start:1313 End:1322
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| CTCACCACTTCTAGCCCGTC | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| GCCCATTTCTTTCCACTTCA | |||||||||||||
| Tm_F_1 | 59.867 | Size_F_1 | 20 | Tm_R_1 | 60.051 | Size_R_1 | 20 | PRODUCT Size | 137 bp | Primer Start | 1230 | Primer End | 1366 |
| FORWARD_PRIMER_2 | |||||||||||||
| CTCACCACTTCTAGCCCGTC | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| GGCCGGGTTAAAAAGAACTC | |||||||||||||
| Tm_F_2 | 59.867 | Size_F_2 | 20 | Tm_R_2 | 59.945 | Size_R_2 | 20 | PRODUCT Size | 239 bp | Primer Start | 1230 | Primer End | 1468 |
| FORWARD_PRIMER_3 | |||||||||||||
| TTAATCGATGACCCCCGTTA | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| GCCCATTTCTTTCCACTTCA | |||||||||||||
| Tm_F_3 | 60.146 | Size_F_3 | 20 | Tm_R_3 | 60.051 | Size_R_3 | 20 | PRODUCT Size | 160 bp | Primer Start | 1207 | Primer End | 1366 |