Primers Available
Organism:Festuca pratensis plastid
Repeat:(A)13
Start:20658 End:20670
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| CCGAGTCACTATGGGCGTAT | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| GGAACAACCTGCTCTTCCTG | |||||||||||||
| Tm_F_1 | 59.978 | Size_F_1 | 20 | Tm_R_1 | 59.844 | Size_R_1 | 20 | PRODUCT Size | 266 bp | Primer Start | 20511 | Primer End | 20776 |
| FORWARD_PRIMER_2 | |||||||||||||
| CCGAGTCACTATGGGCGTAT | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| AGCTGGAACAACCTGCTCTT | |||||||||||||
| Tm_F_2 | 59.978 | Size_F_2 | 20 | Tm_R_2 | 59.09 | Size_R_2 | 20 | PRODUCT Size | 270 bp | Primer Start | 20511 | Primer End | 20780 |
| FORWARD_PRIMER_3 | |||||||||||||
| TGTGGGGATCCATAGAGAGC | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| TCCCATGCAATAGTCAGGAA | |||||||||||||
| Tm_F_3 | 60.034 | Size_F_3 | 20 | Tm_R_3 | 59.075 | Size_R_3 | 20 | PRODUCT Size | 208 bp | Primer Start | 20608 | Primer End | 20815 |