Primers Available
Organism:Festuca ovina plastid
Repeat:(A)10
Start:3569 End:3578
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| GGCCAATTGCTCTTTTGACT | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| GCTTGGCTCCAATTCTGGTA | |||||||||||||
| Tm_F_1 | 59.316 | Size_F_1 | 20 | Tm_R_1 | 60.214 | Size_R_1 | 20 | PRODUCT Size | 273 bp | Primer Start | 3442 | Primer End | 3714 |
| FORWARD_PRIMER_2 | |||||||||||||
| AGGCCAATTGCTCTTTTGAC | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| GCTTGGCTCCAATTCTGGTA | |||||||||||||
| Tm_F_2 | 59.316 | Size_F_2 | 20 | Tm_R_2 | 60.214 | Size_R_2 | 20 | PRODUCT Size | 274 bp | Primer Start | 3441 | Primer End | 3714 |
| FORWARD_PRIMER_3 | |||||||||||||
| CACAGATCTTTTTCCGCTCC | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| GGGAAAGATTGGGTTTTCCT | |||||||||||||
| Tm_F_3 | 59.813 | Size_F_3 | 20 | Tm_R_3 | 59.261 | Size_R_3 | 20 | PRODUCT Size | 257 bp | Primer Start | 3353 | Primer End | 3609 |