Primers Available
Organism:Sarcocheilichthys parvus mitochondrion
Repeat:(C)10
Start:538 End:547
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| TTAGCAAAACCCACCTGACC | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| GGTGAGGTTTAACGGGGATT | |||||||||||||
| Tm_F_1 | 59.971 | Size_F_1 | 20 | Tm_R_1 | 60.053 | Size_R_1 | 20 | PRODUCT Size | 213 bp | Primer Start | 455 | Primer End | 667 |
| FORWARD_PRIMER_2 | |||||||||||||
| TTAGCAAAACCCACCTGACC | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| GGGTTTTAAGCTGATGCTCG | |||||||||||||
| Tm_F_2 | 59.971 | Size_F_2 | 20 | Tm_R_2 | 59.845 | Size_R_2 | 20 | PRODUCT Size | 139 bp | Primer Start | 455 | Primer End | 593 |
| FORWARD_PRIMER_3 | |||||||||||||
| AGCTGAGGAACAAACTGGGA | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| GGTGAGGTTTAACGGGGATT | |||||||||||||
| Tm_F_3 | 59.844 | Size_F_3 | 20 | Tm_R_3 | 60.053 | Size_R_3 | 20 | PRODUCT Size | 186 bp | Primer Start | 482 | Primer End | 667 |