Primers Available
Organism:Sarcocheilichthys parvus mitochondrion
Repeat:(AACA)3
Start:13499 End:13510
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| AAAATGGCAGCCCTCCTAGT | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| GAATCATGTCCGGTCGAGTT | |||||||||||||
| Tm_F_1 | 60.096 | Size_F_1 | 20 | Tm_R_1 | 59.934 | Size_R_1 | 20 | PRODUCT Size | 216 bp | Primer Start | 13436 | Primer End | 13651 |
| FORWARD_PRIMER_2 | |||||||||||||
| AAAATGGCAGCCCTCCTAGT | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| GATGAATAATCGCGGGAAAA | |||||||||||||
| Tm_F_2 | 60.096 | Size_F_2 | 20 | Tm_R_2 | 59.87 | Size_R_2 | 20 | PRODUCT Size | 148 bp | Primer Start | 13436 | Primer End | 13583 |
| FORWARD_PRIMER_3 | |||||||||||||
| CCGTAACCCTAAAAATGGCA | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| GAATCATGTCCGGTCGAGTT | |||||||||||||
| Tm_F_3 | 59.826 | Size_F_3 | 20 | Tm_R_3 | 59.934 | Size_R_3 | 20 | PRODUCT Size | 227 bp | Primer Start | 13425 | Primer End | 13651 |