Primers Available
Organism:Fragaria mandshurica plastid
Repeat:(C)10
Start:5971 End:5980
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| TGGAAATTTTCTCGAATGGG | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| AAGTGCTCTTGGCTCGACAT | |||||||||||||
| Tm_F_1 | 59.872 | Size_F_1 | 20 | Tm_R_1 | 60.02 | Size_R_1 | 20 | PRODUCT Size | 222 bp | Primer Start | 5813 | Primer End | 6034 |
| FORWARD_PRIMER_2 | |||||||||||||
| AAACCTTCCCTTGGAATTGG | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| AAGTGCTCTTGGCTCGACAT | |||||||||||||
| Tm_F_2 | 60.159 | Size_F_2 | 20 | Tm_R_2 | 60.02 | Size_R_2 | 20 | PRODUCT Size | 239 bp | Primer Start | 5796 | Primer End | 6034 |
| FORWARD_PRIMER_3 | |||||||||||||
| TCTTGCCCCTGAAAAAGAAA | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| AAGTGCTCTTGGCTCGACAT | |||||||||||||
| Tm_F_3 | 59.795 | Size_F_3 | 20 | Tm_R_3 | 60.02 | Size_R_3 | 20 | PRODUCT Size | 129 bp | Primer Start | 5906 | Primer End | 6034 |