Primers Available
Organism:Fragaria mandshurica plastid
Repeat:(A)15gaat(A)11
Start:8636 End:8665
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| AGGGGCTTTTTAGTTCCACG | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| TGGCAAGAAACGAGAGTGC | |||||||||||||
| Tm_F_1 | 60.474 | Size_F_1 | 20 | Tm_R_1 | 60.128 | Size_R_1 | 19 | PRODUCT Size | 280 bp | Primer Start | 8539 | Primer End | 8818 |
| FORWARD_PRIMER_2 | |||||||||||||
| CGAAGGGGCTTTTTAGTTCC | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| AAACGAGAGTGCCCTTTTTC | |||||||||||||
| Tm_F_2 | 60.068 | Size_F_2 | 20 | Tm_R_2 | 58.436 | Size_R_2 | 20 | PRODUCT Size | 276 bp | Primer Start | 8536 | Primer End | 8811 |
| FORWARD_PRIMER_3 | |||||||||||||
| CCGAAGGGGCTTTTTAGTTC | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| AAACGAGAGTGCCCTTTTTC | |||||||||||||
| Tm_F_3 | 60.068 | Size_F_3 | 20 | Tm_R_3 | 58.436 | Size_R_3 | 20 | PRODUCT Size | 277 bp | Primer Start | 8535 | Primer End | 8811 |