Primers Available
Organism:Puntius chalakkudiensis mitochondrion
Repeat:(TTA)4
Start:10758 End:10769
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| AACCAATTAACCGACAACGC | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| CTGAGCCGTTCAGTTTGGTT | |||||||||||||
| Tm_F_1 | 59.867 | Size_F_1 | 20 | Tm_R_1 | 60.291 | Size_R_1 | 20 | PRODUCT Size | 172 bp | Primer Start | 10628 | Primer End | 10799 |
| FORWARD_PRIMER_2 | |||||||||||||
| AACCAATTAACCGACAACGC | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| TGTTGGAGTAGAAGCAGGGC | |||||||||||||
| Tm_F_2 | 59.867 | Size_F_2 | 20 | Tm_R_2 | 60.397 | Size_R_2 | 20 | PRODUCT Size | 247 bp | Primer Start | 10628 | Primer End | 10874 |
| FORWARD_PRIMER_3 | |||||||||||||
| CAATTAACCGACAACGCTCA | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| TGTTGGAGTAGAAGCAGGGC | |||||||||||||
| Tm_F_3 | 59.729 | Size_F_3 | 20 | Tm_R_3 | 60.397 | Size_R_3 | 20 | PRODUCT Size | 244 bp | Primer Start | 10631 | Primer End | 10874 |