Primers Available
Organism:Puntius chalakkudiensis mitochondrion
Repeat:(T)11
Start:16553 End:16563
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| CCTGGCATTTGGTTAATGGT | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| TTGCACTCTGAAATGCGAAG | |||||||||||||
| Tm_F_1 | 59.685 | Size_F_1 | 20 | Tm_R_1 | 60.134 | Size_R_1 | 20 | PRODUCT Size | 120 bp | Primer Start | 16475 | Primer End | 16594 |
| FORWARD_PRIMER_2 | |||||||||||||
| CCTGGCATTTGGTTAATGGT | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| ACGCGTGAAAACCTGAAAGT | |||||||||||||
| Tm_F_2 | 59.685 | Size_F_2 | 20 | Tm_R_2 | 59.78 | Size_R_2 | 20 | PRODUCT Size | 231 bp | Primer Start | 16475 | Primer End | 16705 |
| FORWARD_PRIMER_3 | |||||||||||||
| AATAATTGTGGGGGTTGCAC | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| TTGCACTCTGAAATGCGAAG | |||||||||||||
| Tm_F_3 | 59.551 | Size_F_3 | 20 | Tm_R_3 | 60.134 | Size_R_3 | 20 | PRODUCT Size | 213 bp | Primer Start | 16382 | Primer End | 16594 |