Primers Available
Organism:Cynotilapia afra mitochondrion
Repeat:(T)10
Start:16214 End:16223
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| CATACTCCTCGTTACCCACCA | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| AAACCGGGGGTGATAATAGG | |||||||||||||
| Tm_F_1 | 59.861 | Size_F_1 | 21 | Tm_R_1 | 59.903 | Size_R_1 | 20 | PRODUCT Size | 255 bp | Primer Start | 16157 | Primer End | 16411 |
| FORWARD_PRIMER_2 | |||||||||||||
| TTCATCGACGCTTGCATAAG | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| TGCAAGGAAAATGTTCAACC | |||||||||||||
| Tm_F_2 | 59.976 | Size_F_2 | 20 | Tm_R_2 | 58.599 | Size_R_2 | 20 | PRODUCT Size | 166 bp | Primer Start | 16122 | Primer End | 16287 |
| FORWARD_PRIMER_3 | |||||||||||||
| CTTTCATCGACGCTTGCATA | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| TGCAAGGAAAATGTTCAACC | |||||||||||||
| Tm_F_3 | 59.976 | Size_F_3 | 20 | Tm_R_3 | 58.599 | Size_R_3 | 20 | PRODUCT Size | 168 bp | Primer Start | 16120 | Primer End | 16287 |