Primers Available
Organism:Stichopathes lutkeni mitochondrion
Repeat:(T)10
Start:17246 End:17255
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| ATGGGCCCAAAAGGAATATC | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| GCAGGACTTGAACCCACACT | |||||||||||||
| Tm_F_1 | 59.984 | Size_F_1 | 20 | Tm_R_1 | 60.159 | Size_R_1 | 20 | PRODUCT Size | 253 bp | Primer Start | 17067 | Primer End | 17319 |
| FORWARD_PRIMER_2 | |||||||||||||
| CTTATGAACAGTCTGGCCCC | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| GCAGGACTTGAACCCACACT | |||||||||||||
| Tm_F_2 | 59.55 | Size_F_2 | 20 | Tm_R_2 | 60.159 | Size_R_2 | 20 | PRODUCT Size | 134 bp | Primer Start | 17186 | Primer End | 17319 |
| FORWARD_PRIMER_3 | |||||||||||||
| ATTAATGGGCCCAAAAGGAA | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| GCAGGACTTGAACCCACACT | |||||||||||||
| Tm_F_3 | 60.489 | Size_F_3 | 20 | Tm_R_3 | 60.159 | Size_R_3 | 20 | PRODUCT Size | 257 bp | Primer Start | 17063 | Primer End | 17319 |