Primers Available
Organism:Lentinula edodes mitochondrion
Repeat:(TTTATT)5
Start:25371 End:25400
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| GAATCTGAAATTGTTTCTGAGGCT | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| AAGGGTGTGGATCTCCTCCT | |||||||||||||
| Tm_F_1 | 60.134 | Size_F_1 | 24 | Tm_R_1 | 59.929 | Size_R_1 | 20 | PRODUCT Size | 215 bp | Primer Start | 25305 | Primer End | 25519 |
| FORWARD_PRIMER_2 | |||||||||||||
| GAATCTGAAATTGTTTCTGAGGCT | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| ATGGGTGCGGAGATAGAATG | |||||||||||||
| Tm_F_2 | 60.134 | Size_F_2 | 24 | Tm_R_2 | 59.917 | Size_R_2 | 20 | PRODUCT Size | 189 bp | Primer Start | 25305 | Primer End | 25493 |
| FORWARD_PRIMER_3 | |||||||||||||
| GAATCTGAAATTGTTTCTGAGGCT | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| CCTAAATGGGTGCGGAGATA | |||||||||||||
| Tm_F_3 | 60.134 | Size_F_3 | 24 | Tm_R_3 | 59.916 | Size_R_3 | 20 | PRODUCT Size | 194 bp | Primer Start | 25305 | Primer End | 25498 |