Primers Available
Organism:Lentinula edodes mitochondrion
Repeat:(ATATTT)4atatatgtatttata(TTATAT)5ttttttaaggaaagcgatcctaaggtaaacctttagtataatttacttcctgaagtaaaacattttatt(AAGGA)3
Start:50019 End:50171
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| AAAAAGGAGGTACTCTACCAAAAGC | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| GCTAATCCGATTTCACTCGC | |||||||||||||
| Tm_F_1 | 59.662 | Size_F_1 | 25 | Tm_R_1 | 59.813 | Size_R_1 | 20 | PRODUCT Size | 232 bp | Primer Start | 49985 | Primer End | 50216 |
| FORWARD_PRIMER_2 | |||||||||||||
| AAAAAGGAGGTACTCTACCAAAAGC | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| TCGCCATTACTTTCGGAGTC | |||||||||||||
| Tm_F_2 | 59.662 | Size_F_2 | 25 | Tm_R_2 | 60.214 | Size_R_2 | 20 | PRODUCT Size | 216 bp | Primer Start | 49985 | Primer End | 50200 |
| FORWARD_PRIMER_3 | |||||||||||||
| AAAAGGAGGTACTCTACCAAAAGC | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| GCTAATCCGATTTCACTCGC | |||||||||||||
| Tm_F_3 | 58.534 | Size_F_3 | 24 | Tm_R_3 | 59.813 | Size_R_3 | 20 | PRODUCT Size | 231 bp | Primer Start | 49986 | Primer End | 50216 |