Primers Available
Organism:Lentinula edodes mitochondrion
Repeat:(AACAAA)4
Start:677 End:700
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| ACACCATCGTGAAGGTCCTC | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| CAGGTTCAGGCGAATCAACT | |||||||||||||
| Tm_F_1 | 59.969 | Size_F_1 | 20 | Tm_R_1 | 60.255 | Size_R_1 | 20 | PRODUCT Size | 233 bp | Primer Start | 538 | Primer End | 770 |
| FORWARD_PRIMER_2 | |||||||||||||
| ACACCATCGTGAAGGTCCTC | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| TTAGCAGGTTCAGGCGAATC | |||||||||||||
| Tm_F_2 | 59.969 | Size_F_2 | 20 | Tm_R_2 | 60.352 | Size_R_2 | 20 | PRODUCT Size | 237 bp | Primer Start | 538 | Primer End | 774 |
| FORWARD_PRIMER_3 | |||||||||||||
| ATCTAGGCTAGGGCTGGAGG | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| CAGGTTCAGGCGAATCAACT | |||||||||||||
| Tm_F_3 | 59.827 | Size_F_3 | 20 | Tm_R_3 | 60.255 | Size_R_3 | 20 | PRODUCT Size | 183 bp | Primer Start | 588 | Primer End | 770 |