Primers Available
Organism:Thelazia callipaeda mitochondrion
Repeat:(T)13gtttaaaagtaatagtttggttttttgttttatgggtttggaaa(T)14cttttggcccggg(T)13ggggagttttattttta(T)17ggaattatgtctagagtttgtggcttggtta(T)11
Start:4872 End:5044
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| TTGAAATTTTGGCTCTTTATCCA | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| AAATAAAACATAACCTTTTCCTTACCA | |||||||||||||
| Tm_F_1 | 59.961 | Size_F_1 | 23 | Tm_R_1 | 58.897 | Size_R_1 | 27 | PRODUCT Size | 274 bp | Primer Start | 4811 | Primer End | 5084 |
| FORWARD_PRIMER_2 | |||||||||||||
| TTGAAATTTTGGCTCTTTATCCA | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| AATAAAACATAACCTTTTCCTTACCA | |||||||||||||
| Tm_F_2 | 59.961 | Size_F_2 | 23 | Tm_R_2 | 57.83 | Size_R_2 | 26 | PRODUCT Size | 273 bp | Primer Start | 4811 | Primer End | 5083 |
| FORWARD_PRIMER_3 | |||||||||||||
| TTGAAATTTTGGCTCTTTATCCA | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| AAAACATAACCTTTTCCTTACCATAA | |||||||||||||
| Tm_F_3 | 59.961 | Size_F_3 | 23 | Tm_R_3 | 57.83 | Size_R_3 | 26 | PRODUCT Size | 270 bp | Primer Start | 4811 | Primer End | 5080 |