Primers Available
Organism:Drosophila pseudoobscura mitochondrion
Repeat:(AT)7
Start:161 End:174
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| GAATTGCCTGATGAAAAGGG | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| TGGGGTATGAACCCAGTAGC | |||||||||||||
| Tm_F_1 | 59.505 | Size_F_1 | 20 | Tm_R_1 | 59.813 | Size_R_1 | 20 | PRODUCT Size | 228 bp | Primer Start | 3 | Primer End | 230 |
| FORWARD_PRIMER_2 | |||||||||||||
| CCTGATGAAAAGGGTCACCT | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| TGGGGTATGAACCCAGTAGC | |||||||||||||
| Tm_F_2 | 58.989 | Size_F_2 | 20 | Tm_R_2 | 59.813 | Size_R_2 | 20 | PRODUCT Size | 222 bp | Primer Start | 9 | Primer End | 230 |
| FORWARD_PRIMER_3 | |||||||||||||
| ATTGCCTGATGAAAAGGGTC | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| TGGGGTATGAACCCAGTAGC | |||||||||||||
| Tm_F_3 | 58.984 | Size_F_3 | 20 | Tm_R_3 | 59.813 | Size_R_3 | 20 | PRODUCT Size | 226 bp | Primer Start | 5 | Primer End | 230 |