Primers Available
Organism:Datura stramonium chloroplast
Repeat:(TTA)4
Start:53314 End:53325
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| ATCTGCTTAAACGATTGCCG | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| GAAAGAGCCCAATGCAAAAA | |||||||||||||
| Tm_F_1 | 60.23 | Size_F_1 | 20 | Tm_R_1 | 60.188 | Size_R_1 | 20 | PRODUCT Size | 245 bp | Primer Start | 53138 | Primer End | 53382 |
| FORWARD_PRIMER_2 | |||||||||||||
| TCGAACCGTAGACCTTCTCG | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| TGAATCAGAGCACATGGAGC | |||||||||||||
| Tm_F_2 | 60.388 | Size_F_2 | 20 | Tm_R_2 | 59.95 | Size_R_2 | 20 | PRODUCT Size | 193 bp | Primer Start | 53277 | Primer End | 53469 |
| FORWARD_PRIMER_3 | |||||||||||||
| TCGAACCGTAGACCTTCTCG | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| GAAAGAGCCCAATGCAAAAA | |||||||||||||
| Tm_F_3 | 60.388 | Size_F_3 | 20 | Tm_R_3 | 60.188 | Size_R_3 | 20 | PRODUCT Size | 106 bp | Primer Start | 53277 | Primer End | 53382 |