Primers Available
Organism:Datura stramonium chloroplast
Repeat:(T)10caagcgtggaaaccccagaaccagaagtagtaggattgattctcataataata(AAAT)3
Start:56229 End:56303
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| AGTACCGGACCGATGATTTG | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| AATTGCATGGTGTCAACGAA | |||||||||||||
| Tm_F_1 | 59.813 | Size_F_1 | 20 | Tm_R_1 | 59.972 | Size_R_1 | 20 | PRODUCT Size | 227 bp | Primer Start | 56192 | Primer End | 56418 |
| FORWARD_PRIMER_2 | |||||||||||||
| AGTACCGGACCGATGATTTG | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| ATTGCATGGTGTCAACGAAA | |||||||||||||
| Tm_F_2 | 59.813 | Size_F_2 | 20 | Tm_R_2 | 59.972 | Size_R_2 | 20 | PRODUCT Size | 226 bp | Primer Start | 56192 | Primer End | 56417 |
| FORWARD_PRIMER_3 | |||||||||||||
| AGTACCGGACCGATGATTTG | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| TTGCATGGTGTCAACGAAAT | |||||||||||||
| Tm_F_3 | 59.813 | Size_F_3 | 20 | Tm_R_3 | 59.972 | Size_R_3 | 20 | PRODUCT Size | 225 bp | Primer Start | 56192 | Primer End | 56416 |