Primers Available
Organism:Datura stramonium chloroplast
Repeat:(T)10
Start:10187 End:10196
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| CCCGCTACCCGCTCTATATC | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| TGCGTCCAATAGGATTTGAA | |||||||||||||
| Tm_F_1 | 60.93 | Size_F_1 | 20 | Tm_R_1 | 59.112 | Size_R_1 | 20 | PRODUCT Size | 250 bp | Primer Start | 10062 | Primer End | 10311 |
| FORWARD_PRIMER_2 | |||||||||||||
| AGCCTTCCAAGCTAACGATG | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| TGGACGCTTTTCATTCTGATT | |||||||||||||
| Tm_F_2 | 59.476 | Size_F_2 | 20 | Tm_R_2 | 59.699 | Size_R_2 | 21 | PRODUCT Size | 216 bp | Primer Start | 10031 | Primer End | 10246 |
| FORWARD_PRIMER_3 | |||||||||||||
| AGCCTTCCAAGCTAACGATG | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| AATGGACGCTTTTCATTCTGA | |||||||||||||
| Tm_F_3 | 59.476 | Size_F_3 | 20 | Tm_R_3 | 59.699 | Size_R_3 | 21 | PRODUCT Size | 218 bp | Primer Start | 10031 | Primer End | 10248 |