Primers Available
Organism:Datura stramonium chloroplast
Repeat:(A)15
Start:115383 End:115397
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| GGGGAGTTTTATTTTCCCCA | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| TCTCTATTGGGCTAAGGGCA | |||||||||||||
| Tm_F_1 | 59.992 | Size_F_1 | 20 | Tm_R_1 | 59.801 | Size_R_1 | 20 | PRODUCT Size | 256 bp | Primer Start | 115333 | Primer End | 115588 |
| FORWARD_PRIMER_2 | |||||||||||||
| TCCCCATCGACCTATTTTCA | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| TCTCTATTGGGCTAAGGGCA | |||||||||||||
| Tm_F_2 | 60.266 | Size_F_2 | 20 | Tm_R_2 | 59.801 | Size_R_2 | 20 | PRODUCT Size | 242 bp | Primer Start | 115347 | Primer End | 115588 |
| FORWARD_PRIMER_3 | |||||||||||||
| ATTCCAAGGTGGGGAGTTTT | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| TCTCTATTGGGCTAAGGGCA | |||||||||||||
| Tm_F_3 | 59.668 | Size_F_3 | 20 | Tm_R_3 | 59.801 | Size_R_3 | 20 | PRODUCT Size | 266 bp | Primer Start | 115323 | Primer End | 115588 |