Primers Available
Organism:Acentrogobius pflaumii mitochondrion
Repeat:(T)11
Start:16177 End:16187
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| ATTATTACTGGCCCCGGTTC | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| GCAAGGAATATGGACACGCT | |||||||||||||
| Tm_F_1 | 60.041 | Size_F_1 | 20 | Tm_R_1 | 60.103 | Size_R_1 | 20 | PRODUCT Size | 225 bp | Primer Start | 16027 | Primer End | 16251 |
| FORWARD_PRIMER_2 | |||||||||||||
| ATTATTACTGGCCCCGGTTC | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| GCTGCACTCTGAAATGCAAA | |||||||||||||
| Tm_F_2 | 60.041 | Size_F_2 | 20 | Tm_R_2 | 60.142 | Size_R_2 | 20 | PRODUCT Size | 188 bp | Primer Start | 16027 | Primer End | 16214 |
| FORWARD_PRIMER_3 | |||||||||||||
| CGACACTCGCATTGACTGTT | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| GCAAGGAATATGGACACGCT | |||||||||||||
| Tm_F_3 | 59.905 | Size_F_3 | 20 | Tm_R_3 | 60.103 | Size_R_3 | 20 | PRODUCT Size | 162 bp | Primer Start | 16090 | Primer End | 16251 |