Primers Available
Organism:Acentrogobius pflaumii mitochondrion
Repeat:(CTCC)3
Start:5768 End:5779
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| GGATTCGGCAACTGACTGAT | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| ATTAATGGCCCCCAGGATAG | |||||||||||||
| Tm_F_1 | 60.081 | Size_F_1 | 20 | Tm_R_1 | 59.999 | Size_R_1 | 20 | PRODUCT Size | 261 bp | Primer Start | 5683 | Primer End | 5943 |
| FORWARD_PRIMER_2 | |||||||||||||
| GGATTCGGCAACTGACTGAT | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| AATTAATGGCCCCCAGGATA | |||||||||||||
| Tm_F_2 | 60.081 | Size_F_2 | 20 | Tm_R_2 | 60.349 | Size_R_2 | 20 | PRODUCT Size | 262 bp | Primer Start | 5683 | Primer End | 5944 |
| FORWARD_PRIMER_3 | |||||||||||||
| GGATTCGGCAACTGACTGAT | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| AGGATAGAGGAGATTCCGGC | |||||||||||||
| Tm_F_3 | 60.081 | Size_F_3 | 20 | Tm_R_3 | 59.634 | Size_R_3 | 20 | PRODUCT Size | 248 bp | Primer Start | 5683 | Primer End | 5930 |