Primers Available
Organism:Panthera leo persica mitochondrion
Repeat:(ACACGT)8
Start:407 End:454
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| TCCCTATTGCTCCACAGGAC | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| CGTGTGTACGTGTGTACGTGT | |||||||||||||
| Tm_F_1 | 60.073 | Size_F_1 | 20 | Tm_R_1 | 58.04 | Size_R_1 | 21 | PRODUCT Size | 265 bp | Primer Start | 220 | Primer End | 484 |
| FORWARD_PRIMER_2 | |||||||||||||
| TCCCTATTGCTCCACAGGAC | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| ACGTGTGTACGTGTGTACGTG | |||||||||||||
| Tm_F_2 | 60.073 | Size_F_2 | 20 | Tm_R_2 | 58.04 | Size_R_2 | 21 | PRODUCT Size | 266 bp | Primer Start | 220 | Primer End | 485 |
| FORWARD_PRIMER_3 | |||||||||||||
| TCCCTATTGCTCCACAGGAC | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| CGTGTGTACGTGTGTACGTGT | |||||||||||||
| Tm_F_3 | 60.073 | Size_F_3 | 20 | Tm_R_3 | 58.04 | Size_R_3 | 21 | PRODUCT Size | 273 bp | Primer Start | 220 | Primer End | 492 |