Primers Available
Organism:Blastocystis sp. subtype 3 mitochondrion
Repeat:(TATT)3
Start:5752 End:5763
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| TTTTAATCGATCGAGTCGGG | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| ATCGTGGATTTCGTTTAGCC | |||||||||||||
| Tm_F_1 | 60.031 | Size_F_1 | 20 | Tm_R_1 | 59.046 | Size_R_1 | 20 | PRODUCT Size | 160 bp | Primer Start | 5678 | Primer End | 5837 |
| FORWARD_PRIMER_2 | |||||||||||||
| ATCCAAAGTGGTTTTCCACG | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| ATCGTGGATTTCGTTTAGCC | |||||||||||||
| Tm_F_2 | 59.83 | Size_F_2 | 20 | Tm_R_2 | 59.046 | Size_R_2 | 20 | PRODUCT Size | 267 bp | Primer Start | 5571 | Primer End | 5837 |
| FORWARD_PRIMER_3 | |||||||||||||
| GGGGTTCAATTCCTCGTACA | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| ATCGTGGATTTCGTTTAGCC | |||||||||||||
| Tm_F_3 | 59.79 | Size_F_3 | 20 | Tm_R_3 | 59.046 | Size_R_3 | 20 | PRODUCT Size | 143 bp | Primer Start | 5695 | Primer End | 5837 |