Primers Available
Organism:Gossypium gossypioides chloroplast
Repeat:(T)13caattgaaaattccc(AAT)4
Start:13494 End:13533
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| CGCTTTGACTCCTTTTCCAT | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| TGCCATTGACATGAAACCTG | |||||||||||||
| Tm_F_1 | 59.312 | Size_F_1 | 20 | Tm_R_1 | 60.517 | Size_R_1 | 20 | PRODUCT Size | 277 bp | Primer Start | 13295 | Primer End | 13571 |
| FORWARD_PRIMER_2 | |||||||||||||
| CGCTTTGACTCCTTTTCCAT | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| GAAACCTGCCCCCTAATTCT | |||||||||||||
| Tm_F_2 | 59.312 | Size_F_2 | 20 | Tm_R_2 | 59.42 | Size_R_2 | 20 | PRODUCT Size | 265 bp | Primer Start | 13295 | Primer End | 13559 |
| FORWARD_PRIMER_3 | |||||||||||||
| CGCTTTGACTCCTTTTCCAT | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| TTTGCCATTGACATGAAACC | |||||||||||||
| Tm_F_3 | 59.312 | Size_F_3 | 20 | Tm_R_3 | 59.375 | Size_R_3 | 20 | PRODUCT Size | 279 bp | Primer Start | 13295 | Primer End | 13573 |