Primers Available
Organism:Gossypium gossypioides chloroplast
Repeat:(C)10
Start:130550 End:130559
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| CAAATACAAATGGGCGATGG | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| AAAGAAAGGATATTGGGCGG | |||||||||||||
| Tm_F_1 | 61.074 | Size_F_1 | 20 | Tm_R_1 | 60.272 | Size_R_1 | 20 | PRODUCT Size | 223 bp | Primer Start | 130516 | Primer End | 130738 |
| FORWARD_PRIMER_2 | |||||||||||||
| GGCGATGGGGAAAATAAGA | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| AAAGAAAGGATATTGGGCGG | |||||||||||||
| Tm_F_2 | 59.848 | Size_F_2 | 19 | Tm_R_2 | 60.272 | Size_R_2 | 20 | PRODUCT Size | 211 bp | Primer Start | 130528 | Primer End | 130738 |
| FORWARD_PRIMER_3 | |||||||||||||
| AACAAATACAAATGGGCGATG | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| AAAGAAAGGATATTGGGCGG | |||||||||||||
| Tm_F_3 | 59.712 | Size_F_3 | 21 | Tm_R_3 | 60.272 | Size_R_3 | 20 | PRODUCT Size | 225 bp | Primer Start | 130514 | Primer End | 130738 |