Primers Available
Organism:Schizothorax biddulphi mitochondrion
Repeat:(AAATA)3
Start:1140 End:1154
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| AGGTGCACTTGGATCAAACC | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| TGCTTTGGTTGGACCTTTTC | |||||||||||||
| Tm_F_1 | 59.973 | Size_F_1 | 20 | Tm_R_1 | 60.088 | Size_R_1 | 20 | PRODUCT Size | 216 bp | Primer Start | 1007 | Primer End | 1222 |
| FORWARD_PRIMER_2 | |||||||||||||
| AGGTGCACTTGGATCAAACC | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| CCGTCTCCCGTACTCAGGTA | |||||||||||||
| Tm_F_2 | 59.973 | Size_F_2 | 20 | Tm_R_2 | 60.126 | Size_R_2 | 20 | PRODUCT Size | 197 bp | Primer Start | 1007 | Primer End | 1203 |
| FORWARD_PRIMER_3 | |||||||||||||
| GAGGCAAGTCGTAACACGGT | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| TGCTTTGGTTGGACCTTTTC | |||||||||||||
| Tm_F_3 | 60.179 | Size_F_3 | 20 | Tm_R_3 | 60.088 | Size_R_3 | 20 | PRODUCT Size | 248 bp | Primer Start | 975 | Primer End | 1222 |