Primers Available
Organism:Helicosporidium sp. ex Simulium jonesi mitochondrion
Repeat:(TTA)4
Start:23856 End:23867
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| AATGCCTGCCTTAATTGGTG | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| AGGATGTTTTCGCATCGAGT | |||||||||||||
| Tm_F_1 | 59.96 | Size_F_1 | 20 | Tm_R_1 | 59.7 | Size_R_1 | 20 | PRODUCT Size | 224 bp | Primer Start | 23737 | Primer End | 23960 |
| FORWARD_PRIMER_2 | |||||||||||||
| AATGCCTGCCTTAATTGGTG | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| TCCCTGCTCCAATTTCAACT | |||||||||||||
| Tm_F_2 | 59.96 | Size_F_2 | 20 | Tm_R_2 | 59.67 | Size_R_2 | 20 | PRODUCT Size | 161 bp | Primer Start | 23737 | Primer End | 23897 |
| FORWARD_PRIMER_3 | |||||||||||||
| AATGCCTGCCTTAATTGGTG | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| GTCCCTGCTCCAATTTCAAC | |||||||||||||
| Tm_F_3 | 59.96 | Size_F_3 | 20 | Tm_R_3 | 59.532 | Size_R_3 | 20 | PRODUCT Size | 162 bp | Primer Start | 23737 | Primer End | 23898 |