Primers Available
Organism:Helicosporidium sp. ex Simulium jonesi mitochondrion
Repeat:(TCCTGC)3
Start:32694 End:32711
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| GCTTCAGTTAAAGCGAACCC | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| GCAAAATTAATCGGAGCAGG | |||||||||||||
| Tm_F_1 | 58.976 | Size_F_1 | 20 | Tm_R_1 | 59.682 | Size_R_1 | 20 | PRODUCT Size | 161 bp | Primer Start | 32589 | Primer End | 32749 |
| FORWARD_PRIMER_2 | |||||||||||||
| GCTTCAGTTAAAGCGAACCC | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| CGGAGCAAAATTAATCGGAG | |||||||||||||
| Tm_F_2 | 58.976 | Size_F_2 | 20 | Tm_R_2 | 59.677 | Size_R_2 | 20 | PRODUCT Size | 165 bp | Primer Start | 32589 | Primer End | 32753 |
| FORWARD_PRIMER_3 | |||||||||||||
| TGCCATCATTAATGCAAACAA | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| GCAAAATTAATCGGAGCAGG | |||||||||||||
| Tm_F_3 | 59.95 | Size_F_3 | 21 | Tm_R_3 | 59.682 | Size_R_3 | 20 | PRODUCT Size | 189 bp | Primer Start | 32561 | Primer End | 32749 |