Primers Available
Organism:Helicosporidium sp. ex Simulium jonesi mitochondrion
Repeat:(ATA)4ttttttctatttaagaagattccccaaaggcctaataagaaaagtatcatagatactgtcataaatttatttaagtccataatatgatt(TTTA)3
Start:12391 End:12503
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| AACGGCTAATAGCATAAGTTCAA | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| AAAAACAACAAGTTTACCTTCAAAA | |||||||||||||
| Tm_F_1 | 57.322 | Size_F_1 | 23 | Tm_R_1 | 57.634 | Size_R_1 | 25 | PRODUCT Size | 212 bp | Primer Start | 12356 | Primer End | 12567 |
| FORWARD_PRIMER_2 | |||||||||||||
| GCGAAAAGTTGACCTAATAAATCAT | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| AAAAACAACAAGTTTACCTTCAAAA | |||||||||||||
| Tm_F_2 | 59.075 | Size_F_2 | 25 | Tm_R_2 | 57.634 | Size_R_2 | 25 | PRODUCT Size | 271 bp | Primer Start | 12297 | Primer End | 12567 |
| FORWARD_PRIMER_3 | |||||||||||||
| AGCGAAAAGTTGACCTAATAAATCAT | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| AAAAACAACAAGTTTACCTTCAAAA | |||||||||||||
| Tm_F_3 | 59.855 | Size_F_3 | 26 | Tm_R_3 | 57.634 | Size_R_3 | 25 | PRODUCT Size | 272 bp | Primer Start | 12296 | Primer End | 12567 |