Primers Available
Organism:Helicosporidium sp. ex Simulium jonesi mitochondrion
Repeat:(AATA)3aatttatattgttctattatacaaaaaatttaaagtataaacaaagcgattacaaataggataaaa(AT)6
Start:6356 End:6445
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| AAGGTATCTTTTTCGTCAGTTTTATTT | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| TGCACCAGTTACAGCACCAG | |||||||||||||
| Tm_F_1 | 58.418 | Size_F_1 | 27 | Tm_R_1 | 60.928 | Size_R_1 | 20 | PRODUCT Size | 234 bp | Primer Start | 6272 | Primer End | 6505 |
| FORWARD_PRIMER_2 | |||||||||||||
| AAGGTATCTTTTTCGTCAGTTTTATTT | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| CTGCACCAGTTACAGCACCA | |||||||||||||
| Tm_F_2 | 58.418 | Size_F_2 | 27 | Tm_R_2 | 60.928 | Size_R_2 | 20 | PRODUCT Size | 235 bp | Primer Start | 6272 | Primer End | 6506 |
| FORWARD_PRIMER_3 | |||||||||||||
| AAAGGTATCTTTTTCGTCAGTTTTATT | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| TGCACCAGTTACAGCACCAG | |||||||||||||
| Tm_F_3 | 58.418 | Size_F_3 | 27 | Tm_R_3 | 60.928 | Size_R_3 | 20 | PRODUCT Size | 235 bp | Primer Start | 6271 | Primer End | 6505 |