Primers Available
Organism:Helicosporidium sp. ex Simulium jonesi mitochondrion
Repeat:(A)10
Start:8528 End:8537
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| TGCATTTCTAAGTTTTCTGCTCT | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| TGTTTTGATTGGTTAGTTTAAAATTG | |||||||||||||
| Tm_F_1 | 57.558 | Size_F_1 | 23 | Tm_R_1 | 57.882 | Size_R_1 | 26 | PRODUCT Size | 279 bp | Primer Start | 8326 | Primer End | 8604 |
| FORWARD_PRIMER_2 | |||||||||||||
| ATGCATTTCTAAGTTTTCTGCTCT | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| TGTTTTGATTGGTTAGTTTAAAATTG | |||||||||||||
| Tm_F_2 | 57.911 | Size_F_2 | 24 | Tm_R_2 | 57.882 | Size_R_2 | 26 | PRODUCT Size | 280 bp | Primer Start | 8325 | Primer End | 8604 |
| FORWARD_PRIMER_3 | |||||||||||||
| TGCATTTCTAAGTTTTCTGCTCTAA | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| TGTTTTGATTGGTTAGTTTAAAATTG | |||||||||||||
| Tm_F_3 | 58.87 | Size_F_3 | 25 | Tm_R_3 | 57.882 | Size_R_3 | 26 | PRODUCT Size | 279 bp | Primer Start | 8326 | Primer End | 8604 |