Primers Available
Organism:Amblyomma elaphense mitochondrion
Repeat:(T)12
Start:13733 End:13744
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| TGAGGGGGATTTTCAGTTGAT | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| TGTTGAAATTTTCTGCGTCTATT | |||||||||||||
| Tm_F_1 | 60.678 | Size_F_1 | 21 | Tm_R_1 | 58.02 | Size_R_1 | 23 | PRODUCT Size | 280 bp | Primer Start | 13532 | Primer End | 13811 |
| FORWARD_PRIMER_2 | |||||||||||||
| GAGGGGGATTTTCAGTTGAT | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| TGTTGAAATTTTCTGCGTCTATT | |||||||||||||
| Tm_F_2 | 57.89 | Size_F_2 | 20 | Tm_R_2 | 58.02 | Size_R_2 | 23 | PRODUCT Size | 279 bp | Primer Start | 13533 | Primer End | 13811 |
| FORWARD_PRIMER_3 | |||||||||||||
| TTGAGGGGGATTTTCAGTTG | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| TTTTCTGCGTCTATTAGATTATAGGGA | |||||||||||||
| Tm_F_3 | 59.903 | Size_F_3 | 20 | Tm_R_3 | 59.939 | Size_R_3 | 27 | PRODUCT Size | 273 bp | Primer Start | 13531 | Primer End | 13803 |