Primers Available
Organism:Huperzia squarrosa mitochondrion
Repeat:(TGAT)3
Start:330768 End:330779
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| CGAGGCCCACTTATTATGGA | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| CAACGCCTGACGTGAAAATA | |||||||||||||
| Tm_F_1 | 59.916 | Size_F_1 | 20 | Tm_R_1 | 59.729 | Size_R_1 | 20 | PRODUCT Size | 248 bp | Primer Start | 330690 | Primer End | 330937 |
| FORWARD_PRIMER_2 | |||||||||||||
| GCGAAGCAATCAATCATCGT | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| CTTTGCCTCCAGTACTTCGC | |||||||||||||
| Tm_F_2 | 61.152 | Size_F_2 | 20 | Tm_R_2 | 60.015 | Size_R_2 | 20 | PRODUCT Size | 255 bp | Primer Start | 330744 | Primer End | 330998 |
| FORWARD_PRIMER_3 | |||||||||||||
| GCGAAGCAATCAATCATCGT | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| AAGCTTGATCTTTGCCTCCA | |||||||||||||
| Tm_F_3 | 61.152 | Size_F_3 | 20 | Tm_R_3 | 59.955 | Size_R_3 | 20 | PRODUCT Size | 264 bp | Primer Start | 330744 | Primer End | 331007 |